| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | Loudonville | A | Wolford | Dessenberg | Fenton | Henley | 48.00 | |
| 4 | Lucas | A | Barrett | Boyd | Walter | Harper | 46.40 | |
| 5 | St. Peter's | A | Duncan | Brubaker | Pruettmiller | Grose | 46.56 | |
| 6 | Crestline | A | Ellenberger | Clark | Silverwood | Reed | 47.60 |